Figure 5
ATF3 expression increases in mouse L4 and L5 DRG neurons after SNIv (cp,t), but not L3. (A) Representative immunofluorescence showing that ATF3 (marker of injured neurons, red) was mostly upregulated in L4 and L5 DRG neurons (HuD positive cells, green) after SNIv(cp,t). Scale bar = 50 μm. (B) Quantification of ATF3-immunoreactivity (IR) in L3, L4 and L5 DRG neurons one week after SNIv(cp,t) or sham surgery. ATF3 was higher in L4 and L5 DRG when the sural nerve was injured, but not in L3. Data are expressed as mean ± SEM, n = 4 animals in each group. ***p < 0.001, two-way ANOVA and post hoc Bonferroni tests. SNIv(cp,t), Spared Nerve Injury variant, sparing common peroneal (cp) and tibial (t) nerves.