Figure 3From: Acid evoked thermal hyperalgesia involves peripheral P2Y1 receptor mediated TRPV1 phosphorylation in a rodent model of thrombus induced ischemic pain Western blot analysis of TRPV1 and phosphorylated TRPV1 (pTRPV1) expression. (A) TRPV1 receptor expression in hind paw skin lysates was quantitatively evaluated in sham + pH 7.0 saline, sham + pH 4.0, thrombus-induced ischemic pain (TIIP) + pH 7.0 saline, acidic saline (pH 4.0) injected TIIP (AS-TIIP) by western blotting. The protein expression of TRPV1 did not change in pH 7.0-treated TIIP and AS- TIIP rat groups compared to 7.0-treated sham groups (n = 4 in each group, 3 days after surgery and pH 4.0 injection). Each band was normalized against the corresponding β-actin band used as loading control. (B) We detected PKC dependent pTRPV1 using western blotting in the hind paw skin of sham + pH 7.0 saline, sham + pH 4.0 saline, TIIP + pH 7.0 saline and AS-TIIP (n = 4 in each group, 3 days after surgery and pH 4.0 saline injections). The expression of pTRPV levels significantly increased in AS-TIIP rats (**p < 0.01 vs sham + pH 7.0 saline) but not in pH 4.0 treated sham and pH 7.0-treated TIIP rats. The pTRPV1 level was normalized against corresponding total TRPV1. Data are presented here as a percentage change (%) compared to the sham control.Back to article page