Figure 6From: Acid evoked thermal hyperalgesia involves peripheral P2Y1 receptor mediated TRPV1 phosphorylation in a rodent model of thrombus induced ischemic pain The Effect of proton sensing ion channels and P2 receptors antagonists in the induction of thermal hyperalgesia (TH) in acidic saline injected TIIP (AS-TIIP) rats. Graphs showing the effects of Amiloride (a ASICs blocker), AMG9810 (a TRPV1 antagonist), TNP-ATP (a P2X antagonist) and MRS2179 (a P2Y1 antagonist) on acidic injection-induced TH in TIIP rats. Each drug was repetitively injected to the hind paw 30 min before pH 4.0 saline injections (D0-3 post-surgery). Amiloride (A, 30 and 100 nmol), AMG9810 (B, 3 and 10 nmol) and TNP-ATP (C, 100 and 300 nmol) given before pH 4.0 saline injection did not prevent the development of TH (n = 7 in each group). Conversely, repetitive treatment with MRS2179 (D, 100 and 300 nmol, n = 4, 6, 5 in each group) effectively prevented the development of TH in the AS-TIIP group (*p < 0.05, **p < 0.01 vs vehicle).Back to article page