Skip to main content

Archived Comments for: A simplified up-down method (SUDO) for measuring mechanical nociception in rodents using von Frey filaments

Back to article

  1. Conversion from Chaplan/Dixon to SUDO scoring

    Robert Bonin, Laval University / IUSMQ

    19 June 2015

    To help researchers currently using the Chapman method switch to SUDO, I've created an Excel spreadsheet that demonstrates code for scoring result strings obtained with Chaplan method (eg., OOOXOXXO) using both the Chaplan/Dixon method and SUDO. The code in this spreadsheet can be used to convert data from Chaplan/Dixon scoring to SUDO. The scoring formulas can also be adapted in your own files for easy data organization and analysis in Excel. 

    The file can be found here: http://bit.ly/1MJhnwd

    Competing interests

    none
  2. Filament Selection and Adjustment Factors

    Robert Bonin, Laval University / IUSMQ

    19 June 2015

    I have had a number of questions about the correct use of the 0.5 "adjustment factor" in SUDO. The adjustment factor is 0.5 x the average spacing of the filament numbering. As long as the spacing of the filament numbering is roughly linear, it is theoretically possible to use any numbering system. We prefer to use whole integers, such as filament number 2, 3, 4, 5, because this is easiest to work with. 

    It is not possible to directly use the bending force of the fibers for calculating the paw withdrawal threshold using SUDO or any other up-down method that involves an "interpolation" between filament numbers to estimate the paw withdrawal threshold. This is because the bending force of the filament increases exponentially as you go up in filament number, making the spacing unequal between different sets of adjacent filaments. 

    This is demonstrated in the "filament adjustment sheet" of this spreadsheet: http://bit.ly/1MJhnwd

    Competing interests

    none

Advertisement