Table 5 List of PCR primers used in this study
Gene | Primer pair (5′→3′) |
|---|---|
CHRNA2 | GTGCCCAACACTTCCGATGT; CCAGCGCAGCTTGTAGTCAT |
CHRNA3 | TCCTGTCATCATCCAGTTTGAGG; TCATTCCAGATTTGCTTCAGCC |
CHRNA4 | CGCTTTGGCTTGTCGATTGC; AGTTTGTAGTCATGCCACTCCT |
CHRNA5 | GAAGGGGCCAGTACGAAAACA; AGCCGAATTTCATGGAGCAAT |
CHRNA6 | TAAAGGCAGTACAGGCTGTGA; AAAATGCACCGTGACGGGAT |
CHRNA7 | CACATTCCACACCAACGTCTT; AAAAGGGAACCAGCGTACATC |
CHRNA9 | GGAACCAGGTGGACATATTCAAT; GCAGCCGTAGGAGATGACG |
CHRNB2 | GAGGTGAAGCACTTCCCATTT; GCCACATCGCTTTTGAGCAC |
CHRNB3 | CAAAGGGGATGAAGGGCAAC; AAAGAGGGTGTAAAACAGGGGC |
CHRNB4 | TGGATGATCTCCTGAACAAAACC; CAGGCGGTAGTCAGTCCATTC |
TRPV1 | TTCCTGCAGAAGAGCAAGAAGC; CCCATTGTGCAGATTGAGCAT |
TRPA1 | GCAGGTGGAACTTCATACCAACT; CACTTTGCGTAAGTACCAGAGTGG |
GAPDH | ATGTGTCCGTCGTGGATCTGA; GCTGTTGAAGTCGCAGGAGACA |