Forebrain overexpression of CaMKII abolishes cingulate long term depression and reduces mechanical allodynia and thermal hyperalgesia
© Wei et al; licensee BioMed Central Ltd. 2006
Received: 14 April 2006
Accepted: 15 June 2006
Published: 15 June 2006
Activity-dependent synaptic plasticity is known to be important in learning and memory, persistent pain and drug addiction. Glutamate NMDA receptor activation stimulates several protein kinases, which then trigger biochemical cascades that lead to modifications in synaptic efficacy. Genetic and pharmacological techniques have been used to show a role for Ca2+/calmodulin-dependent kinase II (CaMKII) in synaptic plasticity and memory formation. However, it is not known if increasing CaMKII activity in forebrain areas affects behavioral responses to tissue injury. Using genetic and pharmacological techniques, we were able to temporally and spatially restrict the over expression of CaMKII in forebrain areas. Here we show that genetic overexpression of CaMKII in the mouse forebrain selectively inhibits tissue injury-induced behavioral sensitization, including allodynia and hyperalgesia, while behavioral responses to acute noxious stimuli remain intact. CaMKII overexpression also inhibited synaptic depression induced by a prolonged repetitive stimulation in the ACC, suggesting an important role for CaMKII in the regulation of cingulate neurons. Our results suggest that neuronal CaMKII activity in the forebrain plays a role in persistent pain.
Ca2+/calmodulin-dependent kinase II (CaMKII) is a key molecule involved in regulating glutamatergic synaptic transmission and learning and memory [1, 2]. Several lines of evidence support a role for CaMKII in synaptic plasticity and memory (see  for review). The ability of CaMKII to autophosphorylate, which prolongs activation in a CaM independent manner, supports an important role for CaMKII in synaptic plasticity . A point mutation in the CaMKII gene that blocks autophosphorylation abolished LTP and impaired spatial memory in mice . While the autophosphorylation and dephosphorylation of CaMKII support the hypothesis that it can act as a "molecular switch" in plasticity and memory, recent studies favor a more sophisticated frequency-dependent biphasic modulation of CaMKII in learning-related synapses. Transgenic mice that overexpress a calcium independent form of CaMKII in forebrain areas demonstrated a shift of frequency-dependent responses to repetitive stimulation . Consistently, these mice displayed an impairment in spatial memory . The role of CaMKII in central synaptic plasticity is not limited to the hippocampus and spatial memory. In the cortex, αCaMKII activity plays a role in long-term or permanent memory and cortical LTP [6, 7].
Glutamatergic synapses involved in sensory transmission can undergo learning-like plastic changes [8–11]. Long-term changes in plasticity along sensory transmission pathways, including the spinal cord dorsal horn and cortical neurons, have been reported [12, 13]. A role for CaMKII in experience-dependent plasticity was shown in the spinal cord dorsal horn, and spinal CaMKII is involved in the development of persistent pain [14, 15]. However, no study has determined if forebrain CaMKII plays a role in the development of persistent pain. The present study takes advantage of recently developed CaMKII transgenic mice that use genetic and chemical techniques to spatially and temporally restrict the expression of CaMKII , We tested if the overexpression of CaMKII in the forebrain affected neuronal properties and synaptic plasticity in the anterior cingulate cortex (ACC), and then carried out behavioral studies to see if responses to peripheral noxious stimuli and tissue injury were affected in forebrain CaMKII overexpressing mice. Synaptic plasticity in the ACC may serve as a cellular model for studying the roles of CaMKII in forebrain structures and behavioral alternations to persistent pain may be due to changes in these forebrain neurons.
CaMKII F89G transgenic male mice (8–12 weeks) were provided by Dr. Tsien's lab (see ). The transgene founders were produced by pronuclear injection of linearized DNA into B6/CBA F1 zygotes, and then intercrossed with B6/CBAF1. The CaMKII F89G transgene expression vector was constructed by inserting the 2.8-kb Not I fragment of the CaMKII F89G transgene into the unique Not I site of pMM279 containing a 8.5-kb CaMKII promotor sequence. Genotyping of the CaMKII F89G transgene was conducted by PCR. Both wild-type and transgenic mice were well groomed and showed no signs of abnormality or any obvious motor defects. As it was impossible to visually distinguish between transgenic and wild-type mice, experimenters were blind to the genotype. The experimental protocols were approved by the Animal Studies Committee at Washington University and University of Toronto.
Transverse slices of the ACC were rapidly prepared and maintained in an interface chamber at 28°C, where they were subfused with artificial cerebrospinal fluid (ACSF) consisting of (in mM) 124 NaCl, 4.4 KCl, 2.0 CaCl2, 1.0 MgSO4, 25 NaHCO3, 1.0 Na2HPO4, and 10 glucose, bubbled with 95% O2 and 5% CO2. Slices were kept in the recording chamber for at least two hr prior to the start of experiments. A bipolar tungsten stimulating electrode was placed in layer V of the ACC, and extracellular field potentials were recorded using a glass microelectrode (3–12 MΩ, filled with ACSF) placed in layer II/III. Five trains of theta burst stimulation (TBS), which consisted of five bursts (four pulses at 100 Hz) of stimuli delivered every 200 ms at the same intensity, were applied to induce LTP. A low-frequency, prolonged stimulation (1 Hz, 15 min) was used to induce LTD. Synaptic responses were elicited at 0.02 Hz. In some experiments, intracellular recordings were also performed with glass microelectrodes filled with 2% Neurobiotin-2 M potassium chloride (80–200 MΩ). After electrophysiological characterization, cells were injected with neurobiotin (+3.0 nA, 150 ms, 3.3 Hz for 5 min). The slices then fixed in 4% paraformaldehyde 30 min after the injection. Morphological procedures were used to stain and identify the recorded cells.
Acute behavioral responses to noxious stimuli
The tail-flick reflex was evoked by focused, radiant heat applied to underside of the tail. The latency to reflexive removal of the tail away from the heat was measured by a photocell timer to the nearest 0.1 sec. The hot-plate (HP) reflex was measured on a metal plate at 55°C (Columbia Instruments). Nociceptive responses included licking or lifting of a hindpaw, or jumping. All mice showed responses within 20 sec. They were removed from the chamber immediately after the first response. In some experiments, the HP response was measured at different temperatures (50 or 52°C). All behavioral tests were performed at 10 min intervals. The baseline response latency was an average of three or four measurements.
Inflammatory pain and measurement of allodynia/hyperalgesia
Formalin (5%, 10 μl) was injected subcutaneously into the dorsal side of a hind paw. The total time spent licking or biting the injected hind paw was recorded and averaged for each 5 min interval over the course of 2 hr. For inflammatory pain, complete Freund's adjuvant (CFA, 50% in saline, 10 μl; Sigma) was injected into the dorsal surface of the left hind paw. After 24 h and 72 h, animals were placed in individual plastic boxes and allowed to adjust to the environment for 1 h. Using the up-down paradigm, mechanical sensitivity was assessed with a set of von Frey filaments (Stoelting; Wood Dale, Illinois) modified so that the filament extended in parallel to the rod. The up-down paradigm method was used to determine the mechanical threshold. For the measurement of mechanical allodynia, based on preliminary experiments that characterized the threshold stimulus, the No. 2.44 filament (0.036 gm force) innocuous for untreated wild-type mouse but representing 50% of the threshold force in animals with injection of CFA, was used to detect mechanical allodynia [16, 18]. The filament was applied to the point of bending 6 times each to the dorsal surfaces of the left and right hindpaws. Positive responses included prolonged hindpaw withdrawal followed by licking or scratching. For each time point, the percent response frequency of hindpaw withdrawal was expressed as (number of positive responses)/6 × 100 per hindpaw.
Heat hyperalgesia was measured by recording the withdrawal latency of a single hind paw from a radiant heat source (IITC Life Sciences instrument, Woodland Hills, Calif). Mice were placed in Plexiglas restrainers on an elevated platform with a clear glass top. A radiant heat source with an "active intensity" of 30 (active intensity is the intensity of light source as coined by the manufacturer) was used as the stimulus. The heat source was positioned on the plantar skin of a hind paw. A built in timer starts when the light beam is switched on. When the animal withdrew its paw abruptly, the heat source and the timer were stopped simultaneously. The duration in seconds from the start of heat application to the paw withdrawal was measured as the paw withdrawal latency.
Pharmacology and drug administration
Chemical synthesis and tritiation of 1-Naphthylmethyl (1NM-PP1) was described previously . Briefly, the ATP-binding pocket of αCaMKII kinase was enlarged via silent mutation. 1NM-PP1 was designed to fit only this enlarged pocket and not the unmodified pocket of native αCaMKII. By using αCaMKII promoter-driven construct, we were able to selectively over express αCaMKII in forebrain neurons. The over expressed αCaMKII-F89G activity could be inhibited by noninvasive oral intake of 1NM-PP1 (5 μM in drinking water) for more than 24 hours (see ). The complete inhibition of αCaMKII-F89G activity can be maintained as long as the 1NM-PP1-containing water is provided. In some experiments, slices from wild-type littermates taking 1NM-PP1-containing drinking water were used as controls.
Data are presented as the mean ± SEM. Statistical comparisons were made with the use of analyses of variance (ANOVAs: Newman-Keuls tests for post hoc comparison) or Student's t-test. P < 0.05 was considered significant.
In general, αCaMKII-F89G transgenic (Tg) mice were visually indistinguishable from wild-type mice. Our previous study used biochemical and pharmacological techniques to screen five different lines of transgenic mice, and the CaMKII Tg-1 line was found to have the highest transgenic mRNA expression. CaMKII overexpression was limited to the forebrain areas, including the ACC, hippocampus, and amygdala, using a αCaMKII promoter-driven construct (see Methods and ). No CaMKII overexpression was detected in the hindbrain or spinal cord . Furthermore, biochemical experiments showed that calcium-dependent CaMKII activity was increased by 2.6 fold in transgenic mice compared to wild-type mice. No changes in other protein kinase such as β CaMKII or CaMKIV were found. Administration of 0.5 μM 1NM-PP1 (a compound that inhibits transgenic CaMKII, see methods) in vitro or systemic application of 1NM-PP1 in Tg-1 mice was found to inhibit overexpressed activity without effecting basal CaMKII in wild-type mice. Thus we chose to use the Tg-1 line of transgenic αCaMKII-F89G mice in the present study.
Our results provide the first evidence that CaMKII activity in the forebrain plays an important role in cingulate synaptic depression and behavioral sensitization related to persistent pain after hindpaw inflammation. While our results do not show a direct correlation between the loss of synaptic LTD in the ACC and a reduction in behavioral sensitization in CaMKII transgenic mice, we suggest that the enhanced forebrain CaMKII activity reduced behavioral sensitization to peripheral injury and that the synaptic function of CaMKII in cingulate neurons may be one mechanism contributing to this behavioral phenotype. Although we focused on the area of the ACC in the current study, we cannot rule out the possible contribution of other forebrain areas such as insular cortex and prefrontal cortex to behavioral changes in the transgenic mice. Our present findings, together with a previous report of increased pain behaviors in forebrain NR2B overexpressing mice (Wei et al, 2001), consistently suggest that behavioral sensitization after tissue injury can be regulated or modulated by neuronal activity within the forebrain areas, including the ACC. Loss of LTD in CaMKII transgenic mice may be responsible for the reduction of nociceptive responses after tissue injury. The rescuing effect of 1NM-PP1 in CaMKII transgenic mice provides strong evidence that the electrophysiological and behavioral changes observed in CaMKII transgenic mice are not due to any developmental changes induced by CaMKII overexpression.
The present results provide strong evidence that enhancing the activity of NMDA receptors or CaMKII does not have the same consequence on persistent pain despite being equally implicated in learning-related synaptic plasticity and behavioral memory. We found that forebrain overexpression of CaMKII did not affect basal nociception and behavioral nociceptive responses to subcutaneous injection of formalin. In our previous study in mice over expressing NMDA NR2B receptors in forebrain regions, we found that late behavioral nociceptive responses to formalin was selectively enhanced in transgenic mice . One possible explanation for such a difference is that the over expressed NMDA NR2B receptors are inactive in normal conditions due to the magnesium blockade of NMDA receptors. However, the enhanced activity of CaMKII would tonically alter the signaling pathways within neurons, including a disruption of normal function and expression of NMDA receptors in the forebrain. Therefore, over expressed CaMKII activity within neurons may have opposite effects on certain outcomes (either at synaptic or behavioral levels) as compared with NMDA receptors.
Why was behavioral sensitization to injury reduced while synaptic depression abolished in the ACC CaMKII transgenic mice? One major hypothesis for the roles of the ACC in pain is that neuronal activity in ACC neurons code the magnitude of pain or pain-related unpleasantness . LTP within the ACC caused by injury or amputation may in part encode abnormal or enhanced pain sensation . The function of synaptic LTD is always thought to be the reversed form of LTP; in the case of memory, LTD is proposed to help to erase old memory and thus allow new memory to form. If we treated plastic changes in the ACC as a memory event caused by injury (e.g., CFA injection in this report), we would predict that loss of LTD in the ACC in CaMKII transgenic mice might abolish the animal's ability to 'erase' such 'bad' or 'painful' memory. Therefore, we would predict that behavioral sensitization would be more likely to be enhanced in CaMKII transgenic mice. By contrast, we found that behavioral sensitization was reduced in CaMKII transgenic mice. It is clear that more experiments are needed in the future to investigate if changes in NMDA receptor expression and function in the ACC and other areas in CaMKII transgenic mice may underlie these results. The roles of cingulate synaptic depression during the development of behavioral sensitization remain to explore.
Considering the important roles of CaMKII in neurodevelopment, it is important to confirm that the phenotypic changes found in brain slices and in nociceptive behaviors can be reversed by simply inhibiting the over expression of CaMKII. In the present study, we found that pretreatment with the inhibitor 1NM-PP1 rescued the loss of behavioral sensitization as well as synaptic depression in ACC slices. Therefore, our studies provide strong evidence for the physiological roles of CaMKII in cingulate neurons, both in terms of selectivity and regional effects.
The present new transgenic mice provide a powerful, and new tool for investigating the roles of protein kinases in the expression or maintenance of behavioral sensitization. Understanding these molecular and synaptic mechanisms hold promise for treating chronic pain in patients.
Supported by grants from the EJLB-CIHR Michael Smith Chair in Neurosciences and Mental Health, Canada Research Chair, Canada Institutes for Health Research and NIH NINDS NS42722 to M.Z., NIH-A14 to K.M.S.
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