Figure 2
From: Spinal NKCC1 blockade inhibits TRPV1-dependent referred allodynia
Time course of NKCC1 inhibitor bumetanide blockade of intracolonic capsaicin-evoked referred allodynia. Bumetanide (BUM) or vehicle (VEH) was injected intrathecally (IT) 5 min prior to intracolonic (IC) capsaicin (A) or 20 min (B) or 4 hrs (C) after IC capsaicin. Response frequencies to 1 mN von Frey hair application to the abdomen are shown at the indicated time points post IC capsaicin. Stars (*) indicate significant effects of BUM vs. VEH (two-way anova) while number signs (#) indicate significant effects vs. baseline (BL) at a given time point (one-way anova, # or * p < 0.05, ## or ** p < 0.01, ### or *** p < 0.001; n = 6 per group).