Figure 2
Effects of SNAP on K ATP channel activity and intracellular ATP concentrations in DRG neurons. (A) Representative current traces in SS or SNL neurons recorded in cell-attached configuration at a holding potential of -60 mV. In SS and SNL neurons, bath application of NO donor, SNAP (100 μM), activated KATP channels. This channel activity was inhibited by glybenclamide (1 μM), a specific KATP channel blocker. Arrows indicate closed channel state. (B) Time-dependent changes of SNAP-induced channel activity (NPo) in cell-attached recording, indicating the elapsed time to peak effect. Each point represents measurements from 8 patches (mean ± SD). (C) The concentration-dependent KATP channel activation (NPo enhancement) induced by increasing SNAP concentrations in SS (open circle) and SNL (closed circle) neurons. Each point represents measurements from 8–9 patches (mean ± SD). (D) Intracellular ATP concentration measured by a luciferin-luciferase assay. Cells were stimulated for 10 min in the presence or absence of SNAP. Values are shown as mean ± SD (n = 5–6).