Figure 4
Effects of 8-Br-cGMP on K ATP channel activity in DRG neurons. (A) Representative current traces in SS and SNL neurons recorded from cell-attached configuration at a holding potential of -60 mV. Bath application of 8-Br-cGMP, a membrane-permeable analogue of cGMP, increased KATP current in either SS or SNL neurons. Arrows indicate closed channel state. (B) Time dependent of 8-Br-cGMP-induced channel activation (NPo) during cell-attached recording, indicating the elapsed time to peak effect. Each point represents 8 SS and 7 SNL neurons. (C) Additional effect of 8-Br-cGMP on SNAP (100 μM)-induced steady-state KATP currents in SS neurons. Changes of NPo values in either SS or SNL neurons are summarized in (D). Each vertical bar represents measurements from 5 patches (mean ± SD).