Figure 5
Effects of NO/cGMP/PKG pathway inhibitors on NO-induced K ATP channel activity in DRG neurons. (A) Representative traces of KATP currents in cell-attached patches at -60 mV holding potential from SS or SNL neurons. SNAP (100 μM) activated channel opening (upper traces). Activating effect of SNAP was not inhibited by pretreatment for at least 20 min with neither ODQ (10 μM), a specific sGC inhibitor (middle traces), or KT5823 (1 μM), a PKG inhibitor (lower traces), in either SS or SNL neurons. Arrows indicate closed channel state. (B) Changes of NPo values obtained during baseline, after application of SNAP (100 μM), or 8-Br-cGMP (100 μM) with or without pretreatment of inhibitor in SS or SNL neurons. Each vertical bar represents measurements from 5–6 patches (mean ± SD).