Figure 1From: Endogenous N-acetylaspartylglutamate (NAAG) inhibits synaptic plasticity/transmission in the amygdala in a mouse inflammatory pain model Prolonged nociceptive behaviors in formalin mice model. (A) Baseline thermal withdrawal latency (TWL) was determined for each mouse prior to treatment. This value was used to establish 100% baseline for that subject. Control groups (saline and naïve) mice showed habituation when retested at 3 and 6 hours. Thermal hypersensitivity (lower TWL relative to baseline as 100%) is observed in the formalin treated mice (n = 15) compared to the control groups (saline-treated n = 11; naïve n = 10) at 1 and 3 but not 6 hours post injection. The formalin treated group TWL was significantly different at 1 hr compared to naïve (p < 0.001) and (p < 0.005) saline groups; at 3 hr compared to saline (p < 0.02) and naïve (p < 0.05) groups. TWL returns to baseline values at 24 hours in the treated groups. #p ≤ 0.05 across group comparison for TWL values by two way RM-ANOVA followed by pairwise Tukey t-test post hoc analysis. (B) Mechanical allodynia, measures as paw withdrawal in response to force of probe in grams, was assessed in the injected hindpaws (ipsilateral = injected paw, IP) and opposite (contralateral, CL) hindpaws. There was a significant difference between the ipsilateral and contralateral footpad (p < 0.02) in the formalin mice. Ipsilateral hindpaw allodynia was significant at 1, 6 and 24 hours post-inflammation relative to the baseline values (n = 15). At 24 hours post-inflammation, the contralateral threshold was significantly (p < 0.05) lower compared to the baseline values in the contralateral footpad. ZJ43 (150 mg/kg; IP, n = 10) administered 30 prior to and 8 hours post formalin injection significantly reduced allodynia in the ipsilateral footpad (∞p ≤ 0.05, ZJ43 (formalin)-IP vs. Formalin-IP mechanical withdrawal threshold values at 24 hr post inflammation). The difference between ZJ43 (formalin)-CL and Formalin-CL did not reach statistical significance (p = 0.13). Two way RM-ANOVA was used followed by pairwise Tukey t-test post hoc analysis. Baseline (B) = probe force that induced paw withdrawal in mice prior to injection of formalin or saline. Data presented as mean ± SEM, *p ≤ 0.05 vs. baseline values; was used followed by pairwise Tukey t-test post hoc analysis.Back to article page