Figure 7

Effects of LY367385 on spontaneous and miniature excitatory and inhibitory postsynaptic currents in CeLC neurons from arthritic rats. (A) Original current traces of spontaneous EPSCs (sEPSC) in an individual CeLC neuron (held at -70 mV) from an arthritic rat before (Predrug) and during LY367385 (10 μM). (B, C) Cumulative distribution analysis of sEPSC frequency (B) and amplitude (C). LY367385 caused a significant shift toward larger inter-event intervals (lower frequency; P < 0.05, Kolmogorov-Smirnov test). LY367385 decreased mean sEPSC frequency (B), but not amplitude (C), significantly (n = 6 neurons, P < 0.01, paired t-test). (D) Current traces of miniature EPSCs (mEPSCs) recorded in the presence of TTX (1 μM) in one CeLC neuron from an arthritic rat before (Predrug) and during LY367385 (10 μM). (E, F) LY367385 (10 μM) had no significant effect on frequency (E) and amplitude (F) of mEPSC (cumulative distribution, P > 0.05, Kolmogorov-Smirnov test; means ± SE, n = 8 neurons, P > 0.05, paired t-test). (G) Current traces of sIPSCs recorded in one CeLC neuron (held at 0 mV) in a brain slice from an arthritic rat before (Predrug) and during LY367385 (10 μM). (H, I) LY367385 (10 μM) increased frequency (H), but not amplitude (I), of sIPSCs significantly (cumulative frequency distribution, P < 0.05, Kolmogorov-Smirnov test; mean frequency, n = 7 neurons, P < 0.01, paired t-test). (J) Current traces of mIPSCs in the presence of TTX (1 μM) in one CeLC neuron from an arthritic rat before (Predrug) and during LY367385 (10 μM). (K, L) LY367385 (10 μM) increased frequency (K), but not amplitude (L), of mIPSCs significantly (cumulative frequency distribution, P < 0.05, Kolmogorov-Smirnov test; mean frequency, n = 7 neurons, P < 0.01, paired t-test). (A, D, G, J) Scale bars, 10 pA, 2 s. (B, C, E, F, H, I, K, L) Bar histograms show means ± SE.