Figure 1

Expression profile of mature microRNAs in DRG and the dorsal horn of the spinal cord during peripheral inflammation. (A) CFA injection induced mechanical hypersensitivity 12 hours, 1, 3 and 7 days post-injection (black bars) compared to the basal values (white bars). Mineral-oil injected mice showed no threshold changes from day 1 to 7 post-injection. (B) In DRG, miR-1 (white bars) and -16 (gray bars) were significantly downregulated under the entire inflammatory period. However, miR-206 (black bars) was down regulated only on day 1 and 7 post-injection. (C) In the spinal dorsal horn there was no change in the early inflammatory process (12 h post-injection) whereas the relative expression of miRs-1, -16 and -206 showed a significant increase at day 1, 3 and 7 post-injection. Bars represent mean ± SEM. # p < 0.001 for CFA injected animals compared to mineral oil injected mice, n = 6 - 8 per group; Student's t-test. * p < 0.05, ** p < 0.01, and *** p < 0.001 for each miRNA of the treated group compared to mineral-oil injected animals and euthanized 12 h post-injection (time 0), n = 6-8 per group; Student's t-test.