Figure 5
From: TRESK channel contribution to nociceptive sensory neurons excitability: modulation by nerve injury
Alkylamide effects on DRG sensory neurons. A. Simultaneous measurement of membrane voltage (top) and intracellular calcium (bottom) in a neuron stimulated with IBA (100 μM). Inset: magnification of the action potentials elicited by IBA application. B. Quantification of intracellular calcium responses to IBA (100 μM) application in DRG neurons in culture (n = 101). Left. Percentage of neurons sensitive or insensitive to IBA application. In each group, the percentage of neurons responding also to capsaicin (Cap; 1 μM) is shown. Right. Intracellular calcium increase (fold-increase vs. resting calcium) elicited by vehicle (Veh) application, IBA (100 μM) or capsaicin (1 μM). C. Representative recordings of intracellular calcium in a neuron responding to IBA and Cap (top), only to IBA (middle) or only to Cap (bottom). D. Soma diameter distribution of DRG neurons sensitive and insensitive to IBA. Black bar shows the mean soma value in each group.