Figure 5
From: Differential roles of galanin on mechanical and cooling responses at the primary afferent nociceptor
Differential role of galanin in a model of neuropathic pain [A] Exogenous c.i.a 100 μM galanin and c.i.a 100 μM Gal2-11 both facilitated mechano-nociceptor responses in the partial saphenous nerve injury model of neuropathic pain (** p < 0.01, *** p < 0.001, Kruskal Wallis test with Dunn’s multiple comparison test, galanin n = 15, Gal2-11 n = 6). [B] In the PSNI neuropathic pain model acetone application to the receptive field of cold sensitive C fibres led to a significant increase in evoked activity (***p < 0.001, Kruskal-Wallis with Dunn’s multiple comparison test). Acetone-induced evoked activity was inhibited by c.i.a 100 μM galanin but was not altered by c.i.a 100 μM Gal2-11 (***p < 0.001, one way ANOVA with post-hoc Bonferroni test, n = 10) [C] Acetone-induced withdrawal behaviours in naïve Gal-OE transgenic mice did not differ from WT controls. 7-days after nerve injury the Gal-OE mice demonstrated a significant reduction in cooling pain like behaviours compared to matched WT mice (*p < 0.01, two way ANOVA with post-hoc Bonferroni test, n = 7).