Figure 4

A single administration of oxaliplatin, but not cisplatin and paclitaxel, enhances AITC-evoked nocifensive behaviors. ( A) Two hours after a single administration of vehicle or oxaliplatin (1, 5 or 10 mg/kg), mice received an intraplantar injection of vehicle ( n = 3) or AITC (0.1%, 20 μl; n = 7–14). ( B) 1, 3 or 7 days after a single administration of vehicle or oxaliplatin (5 mg/kg), mice received an intraplantar injection of AITC (0.1%, 20 μl). n = 5–6. ( C) Two hours after a single administration of vehicle or oxaliplatin (5 mg/kg), mice received an intraplantar injection of vehicle or menthol (1.6 μg, 20 μl). n = 6. ( D) TRPA1^+/+ or TRPA1^−/− mice were intraperitoneally administered vehicle or oxaliplatin (5 mg/kg) 2 h before the menthol-evoked nocifensive behaviors. n = 3–4. ( E) Two hours after a single administration of vehicle or oxaliplatin (5 mg/kg), mice received an intraplantar injection of vehicle or capsaicin (160 μg, 20 μl). n = 6. ( F– G) Two hours after a single administration of oxalate (1.7 mg/kg; F), cisplatin (5 mg/kg; G), paclitaxel (6 mg/kg; H) or vehicle, mice received an intraplantar injection of AITC (0.1%, 20 μl). n = 6–7. AITC- and capsaicin-evoked nocifensive behaviors were measured for 20 min and 5 min, respectively; menthol-evoked nocifensive-like behaviors were scored for 5 min. Data are presented as the means ± S.E.M. * p < 0.05; ** p < 0.01; *** p < 0.001.