Fig. 8
From: The opioid peptide dynorphin A induces leukocyte responses via integrin Mac-1 (αMβ2, CD11b/CD18)
Effect of Dyn A on phagocytosis of latex beads. a, Fluorescent latex beads (1x109/ml) were preincubated with Dyn A (50 μg/ml) for 40 min at 37 °C. Soluble peptide was removed from the beads by high-speed centrifugation. Peptide-coated beads were incubated with adherent IC-21 macrophages for 30 min at 37 °C. Nonphagocytosed beads were removed from cells by washing with PBS. IC-21 cells exposed to control (left) and Dyn A-treated (right) beads are shown. b , Peptide-coated beads were incubated with IC-21 macrophages in the presence of anti-αM mAb M1/70 (20 μg/ml), heparin (20 μg/ml), NIF (2 μg/ml) or naloxone (10 μM). Rat IgG2b was used as an isotype control for anti-αM mAb M1/70. Phagocytosis was quantified for five random fields per well using a 20x objective. Phagocytosis of Dyn A-treated beads was assigned as 100 %. Data shown are mean ± S.E. from three separate experiments. **p < 0.01; ns, no significant difference