Figure 3

Postnatal reduction of the axonal branching of EGFP-positive fibers in the dura of TRPM8 ^EGFPf/+ mice. a Representative images of axons containing EGFP-ir in the dura of P2 and adult TRPM8 ^EGFPf/+ mice. Each image contains one fiber. Arrows indicate individual branch points on the fiber. b The average number of EGFP-positive fibers per mm² of mouse dura (n = 5–10 mice in each group, p = 0.17, one-way ANOVA). c The average number of branch points on individual dural EGFP-positive fibers between P2 and adulthood (same mice as in b). *p < 0.05, **p < 0.01, one-way ANOVA with post hoc Bonferroni test, all compared with the P2 group. d The percentage of dural EGFP-positive fibers without branch points between P2 and adulthood (same mice as in b). *p < 0.05, **p < 0.01, one-way ANOVA with post hoc Bonferroni test, all compared with the P2 group. e The number of branch points per mm of EGFP-positive fibers in P2, P11and adult mouse dura (n = 6–10 mice in each group). **p < 0.01, one-way ANOVA with post hoc Bonferroni test.