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Figure 2 | Molecular Pain

Figure 2

From: Sensitization and translocation of TRPV1 by insulin and IGF-I

Figure 2

Insulin and IGF-I potentiate native TRPV1 currents in DRG neurons. a, Capsaicin (100 nM, applied at 2 min intervals) response was enhanced by insulin (1 μM) under perforated patch conditions. b, Time course of insulin induced potentiation of capsaicin currents (■: 500 nM capsaicin, 20 sec application) (n = 4, P < .01 at 5, 10, and 15 min incubation times). Inset shows the time course for experiment in Fig. 2a. c, Summary graph showing fold increase in TRPV1 currents following 10 min treatment with control (n = 15), insulin (1 μM; n = 11, P < .01) and IGF-I (20 nM; n = 4, P < .01). d, Under confocal microscopy, insulin (1 μM, 2 min) potentiated intracellular Ca2+ rise in response to 500 nM capsaicin (n = 10, P < .05). e, Capsaicin induced single channel current activity recorded at +60 mV was increased by exposure of cell to insulin (1 μM). f, Mean capsaicin induced open probability (Po) in the absence and presence of insulin (n = 5, P < .05). g, Mean current amplitude observed from single channel recordings (n = 5).

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