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Figure 5 | Molecular Pain

Figure 5

From: Pre-injury administration of morphine prevents development of neuropathic hyperalgesia through activation of descending monoaminergic mechanisms in the spinal cord in mice

Figure 5

Prevention of nerve injury-induced increase in PKCγ expression in the spinal dorsal horn by pre-injury morphine. A,B: PKCγ immunoreactivity in the ipsilateral (A) and contralateral (B) sides of spinal dorsal horn of sham-operated mice. C,D: PKCγ-IR in the ipsilateral (C) and contralateral (D) sides of nerve-injured mice at 7 days after nerve injury. E,F: PKCγ-IR following pre-injury administration of morphine (10 mg/kg, s.c.) in ipsilateral (E) and contralateral (F) sides of nerve-injured mice at day 7. G-J: Pre-injury i.t. injection of clonidine (30 nmol) and fluoxetine (30 nmol) also prevented the injury-induced increase in PKCγ expression in spinal dorsal horn. K: Quantification of PKCγ immunoreactive fluorescence in ipsilateral sides of sham operated (sham), nerve-injured (injured), pre-injury morphine (PEM), pre-injury clonidine (PEC) and pre-injury fluoxetine (PEF) treated mice. Quantification of staining intensity was done using Scion imaging software for Macintosh. The data were represented as the ratio of staining intensity between ipsilateral and contralateral sides of each section in the treatment groups. Data were taken from three animals of each treatment group taking three separate sections from each animal. *, # p < 0.05. The scale bar represents 100 μm for all images.

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Molecular Pain

ISSN: 1744-8069

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