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Figure 3 | Molecular Pain

Figure 3

From: Spinal cord NR1 serine phosphorylation and NR2B subunit suppression following peripheral inflammation

Figure 3

Serine phosphorylation of spinal cord NR1 subunits following carrageenan induced hind paw inflammation. A. Time course of serine phosphorylation on NR1 subunits immunoprecipitated by antibodies that recognized all NR1 subunits, N1 containing splice variants or C1 containing splice variants. The western blots at the top are representative experiments where the immunoprecipitated protein was probed with anti-phosphoserine antibodies. The bands migrated at approximately 118 kd. The graph summarizes the band density data from the western blots. Data are means ± SEM of the normalized band density data (NR1: N = 6 rats per time point, N1: N = 5 rats per time point, C1: N = 6 rats per time point). Asterisks indicate p < 0.05 ANOVA followed by Dunnett's test on the raw data. B. Comparison of serine phosphorylation on NR1 subunits from the dorsal and ventral spinal cord four hours following the injection of either saline or carrageenan (6 mg) into the left hind paws. At the top is a representative western blot demonstrating the immunoprecipitated NR1 after it was probed with anti-phosphoserine antibodies. The graph is a summary of the normalized band density data for control animals (N = 5), saline injected animals (N = 5) and carrageenan injected animals (N = 5). Data are means ± SEM. Asterisks indicate p < 0.05 ANOVA followed by Dunnett's test.

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