Figure 5

Phosphorylation of NR2A and NR2B subunits. NR2A and NR2B were immunoprecipitated from rat lumbar spinal before and after carrageenan induced inflammation of the left hind paw. The protein was run on western blots and probed with anti-phosphoserine, anti-phosphothreonine or anti-phosphotyrosine antibodies. Single bands were identified on the western blots that migrated at ~180 kd. A. Phosphoserine on NR2A and NR2B subunits. The western blots at the top demonstrate representative experiments and the graph below presents the mean ± SEM band density for all experiments (NR2A N = 4 rats per time point, NR2B N = 4 rats per time point). ANOVA p > 0.05. B. Phosphothreonine on NR2A and NR2B subunits. The western blots at the top demonstrate representative experiments and the graph below presents the mean ± SEM band density for all experiments (NR2A N = 4 rats per time point, NR2B N = 4 rats per time point). ANOVA p > 0.05. C. Phosphotyrosine on NR2A and NR2B subunits. The western blots at the top demonstrate representative experiments and the graph below presents the mean ± SEM band density for all experiments (NR2A N = 4 rats per time point, NR2B N = 4 rats per time point). ANOVA p > 0.05.