Expression of CREB and phosphorylated-CREB protein in spinal cord and the effect of intrathecal PKC inhibitor, NPC 15473, administration on their expression. Panel A. Western blot bands of CREB and phospho-CREB protein expression. Panel B. Bar graph showing the density of the immunoblot band of CREB protein. (NS, no significance between vehicle treatment vs. capsaicin treatment group with either ACSF- or NPC 15473- treatment). Panel C. Bar graph indicating the density of the immunoblotting band of phospho-CREB protein. (*, p < 0.05; the value from vehicle treatment vs. capsaicin treatment; #, p < 0.05, the value of ACSF-treated vs. NPC 15473-treated animals following capsaicin injection; n = 6 in each group). Open bar, vehicle group; hatched bar, capsaicin group.