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Figure 1 | Molecular Pain

Figure 1

From: Neonatal local noxious insult affects gene expression in the spinal dorsal horn of adult rats

Figure 1

Real-time RT-PCR-determined levels of expression (calculated as R0 using β-actin as internal control) for genes identified by microarray profiling as having significantly altered basal bilateral levels in the LDH of CAR-P3 as compared to their bilateral expressions in the LDH of CONT and CAR-P12 animals (Table 1). A, genes, which were identified by microarrays as having similar expressions in CONT and CARP-12 groups, but changed (up-regulated) in CAR-P3 group. B, genes, which were identified by microarrays as having differences in expression between CONT and CAR-P3 and CAR-P12 groups as well as between CARP-3 and CAR-P12 groups (both when CAR-P3 > CAR-P12 and CAR-P3 < CAR-P12). The data for the LDH ipsilareal to the neonatally-injured paw and the LDH contralateral to that paw are shown separately. Each data-point represents a mean of 5 animals in a group. The data were analyzed using one-way ANOVAs, (with the Benjamini and Hochberg's FDR correction procedure set at the P-value cutoff of 0.05) followed by Tukey's post-hoc tests (with the same P-cutoff value) in the implementation of GeneSpring GX software (Agilent, Palo Alto, CA). The presented real-time RT-PCR data support the microarray-based findings except when marked by '$' sign (microarrays suggested CARP-3 expression > CARP12 expression, while RT-PCR showed CAR-P3 expression = CAR-P12 expression) and '&' sign (in contrast to microarrays, no inter-group differences were detected by RT-PCR).

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