Figure 2

Real-time RT-PCR-determined levels of expression (calculated as R₀ using β-actin as internal control) for genes identified by having significantly altered basal levels in the left LDH ipsilateral to the neonatally-injured hindpaw in CAR-P3 as compared to their expressions in the corresponding LDH of CONT and CAR-P12 animals (Table 2). The data for the LDH ipsilareal to the neonatally-injured paw and the LDH contralateral to that paw are shown separately. Each data-point represents a mean of 5 animals in a group. The data were analyzed using one-way ANOVAs, (with the Benjamini and Hochberg's FDR correction procedure set at the P-value cutoff of 0.05) followed by Tukey's post-hoc tests (with the same P-cutoff value) in the implementation of GeneSpring GX software (Agilent, Palo Alto, CA). The presented real-time RT-PCR data support the microarray-based findings.