Figure 3

Real-time RT-PCR-determined levels of expression (calculated as R₀ using β-actin as internal control) for genes identified by microarray profiling as showing significant adult inflammation-induced alterations in their expressions in the left LDH (ipsilateral to the CFA-inflammed neonatally-injured hindpaw) in CAR-P3 rats as compared to those in the left LDH of CONT and CAR-P12 animals after inflammation of the corresponding paw (Table 3). A, genes, which were identified by microarrays as having similar expressions in CONT and CARP-12 groups, but changed (up-regulated) in CAR-P3 group. B, genes, which were identified by microarrays as having differences in expression between CONT and CAR-P3 and CAR-P12 groups as well as between CARP-3 and CAR-P12 groups (both when CAR-P3 > CAR-P12 and CAR-P3 < CAR-P12). Each data-point represents a mean of 5 animals in a group. The data were analyzed using one-way ANOVAs, (with the Benjamini and Hochberg's FDR correction procedure set at the P-value cutoff of 0.05) followed by Tukey's post-hoc tests (with the same P-cutoff value) in the implementation of GeneSpring GX software (Agilent, Palo Alto, CA). The presented real-time RT-PCR data support the microarray-based findings except when marked by '$' sign (microarrays suggested CARP-3 expression > CARP12 expression, while RT-PCR showed CAR-P3 expression = CAR-P12 expression).