Table 1 Summary of primer sequences and antibodies used. The sequence of forward (F) and reverse (R) primers used in PCR experiments are provided along with product sizes. Also provided are the sources for antibodies used and the dilution ranges.
From: Formalin injection causes a coordinated spinal cord CO/NO-cGMP signaling system response
Gene | Primer Sequences | Product Size | Antibody Source, Cat# | Dilution Range |
|---|---|---|---|---|
HO-1 | F:ACGCATATACCCGCTACCTG | 227 | Oncogene, PC340 | 1:100–1:500 |
| Â | R:GAAGGCGGTCTTAGCCTCTT | Â | Â | Â |
HO-2 | F:ACTGAAGAAGGTTGCCCAGA | 179 | Santa Cruz, SC17786 | 1:100–1:500 |
| Â | R:CTTTATTGGCCTCCTCCACA | Â | Â | Â |
nNOS | F:TCAGTCTCCCAGGCTAATGG | 200 | Santa Cruz, SC5302 | 1:200–1:1000 |
| Â | R:CTGTCCACCTGGATTCCTGT | Â | Â | Â |
iNOS | F:CTCACTGGGACAGCACAGAA | 199 | Santa Cruz, SC650 | 1:200–1:500 |
| Â | R:TGGTCAAACTCTTGGGGTTC | Â | Â | Â |
eNOS | F:CTCACTGGGACAGCACAGAA | 199 | Santa Cruz, SC8311 | 1:200–1:1000 |
| Â | R:TGGTCAAACTCTTGGGGTTC | Â | Â | Â |
sGCα1 | F:AGCGACTGAACCTTGCACTT | 119 | Sigma, G4280 | 1:1000–1:2000 |
| Â | R:ACCTGCTGCAATTGCTTCTT | Â | Â | Â |
sGCα2 | F:CGAAAGCAACTTCGATGTGA | 120 | Santa Cruz, SC20954 | 1:100–1:400 |
| Â | R:AAATGGGGTGGACAATCGTA | Â | Â | Â |
PKG Iα | F:AAGCATGATGGGAAAACAGG | 184 | Santa Cruz SC10335 | 1:100–1:400 |
| Â | R:GTGACTGCTGGCTTGTGGTA | Â | Â | Â |
PKG Iβ | F:GACAGCTGCATCATCAAGGA | 198 | Stressgen KAP-PK002 | 1:500–1:1500 |
| Â | R:GATGGCCCAGAGTTTCACAT | Â | Â | Â |
PKG II | F:TGAACCGTGACGATGAAAAA | 186 | Santa Cruz SC25430 | 1:200–1:500 |
| Â | R:CAAGCTCCACTCTTCCGAAC | Â | Â | Â |
PDE 2 | F:TTCAAGCTGCTGCAAGAAGA | 224 | Santa Cruz SC17227 | 1:200–1:1000 |
| Â | R:TTCCTGAGGACCTGGATACG | Â | Â | Â |
PDE 3 | F:TTGCATAATTCAATGCCAAG | 143 | Multiple | Multiple |
| Â | R:TAGGTCCCGATCTTTTGCTG | Â | Â | Â |
PDE 5 | F:AAATGGTGGGACCTTCACTG | 201 | Cell Signaling 4072 | 1:200–1:1000 |
| Â | R:GTGGCCGCTATCTTCTTCAG | Â | Â | Â |