Figure 3

Block of ATP-induced synaptic responses by P2 antagonists A. The histogram shows the effects of ATP on sEPSC frequency in a lamina I neuron in the absence and presence of suramin (30 μM) or PPADS (10 μM). Suramin and PPADS were pre-applied for 10 min and were present during the recording. After 30 min washout of antagonists, 100 μM ATP was applied again for a test of recovery. B. Summarized data show effects of ATP on sEPSC frequency (filled bars) and amplitude (open bars) in the absence (n = 5) and presence of PPADS (10 μM, n = 4) and suramin (30 μM, n = 3). C. Histogram on the left shows a time course of ATP-induced increase of sEPSC frequency in a lamina I neuron in the presence of 100 μM reactive blue 2(RB2). Bar graph on the right is a summary that shows the effects of 100 μM ATP on the sEPSCs in the absence and presence of 100 μM RB2 (n = 11).