Loss of LFS-evoked LTD in the deep layer of the IC after tail amputation. (A) Pooled data of LFS-elicited LTD in the deep layer of the IC for sham control (n = 9 slices/7 mice) and tail amputation (n = 9 slices/5 mice) groups. Tail amputation also abolished the LTD induction in the deep layer. Sample fEPSP recordings taken at the times indicated by the corresponding numbers are shown above the plot. Calibration: 100 μV, 10 ms. Horizontal bars denote the period of LFS delivery. (B) Bar histogram showing the induction ratio of LTD (the percentage of LTD-showing channels among all activated channels) in the superficial layer and deep layer of the IC for sham control (n = 9 slices/7 mice) and tail-amputated (n = 9 slices/5 mice) groups. ***P < 0.001. Error bars represent SEM.