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Figure 2 | Molecular Pain

Figure 2

From: Alleviation of behavioral hypersensitivity in mouse models of inflammatory pain with two structurally different casein kinase 1 (CK1) inhibitors

Figure 2

TG003 inhibits CK1δ/ϵ-induced nuclear translocation of PER3. (A, B) Localization of mCherry-PER3 in HEK293 cells expressing CK1δ (A) or CK1ϵ (B). Prior to doxycycline (Dox)-induced expression of CK1δ/ϵ for 8 hours, the cells were treated with vehicle control, TG003, TG001, or PF-670462 for 1 hour. The treated cells were fixed and stained with Hoechst33342 to define nucleus. Representative images are shown. (C, D). Quantification of the nuclear/cytoplasmic fluorescence intensity ratio. The data are mean ± SEM (n = 20). # P < 0.0001 (Student’s t-test).

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