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Figure 3 | Molecular Pain

Figure 3

From: Acid evoked thermal hyperalgesia involves peripheral P2Y1 receptor mediated TRPV1 phosphorylation in a rodent model of thrombus induced ischemic pain

Figure 3

Western blot analysis of TRPV1 and phosphorylated TRPV1 (pTRPV1) expression. (A) TRPV1 receptor expression in hind paw skin lysates was quantitatively evaluated in sham + pH 7.0 saline, sham + pH 4.0, thrombus-induced ischemic pain (TIIP) + pH 7.0 saline, acidic saline (pH 4.0) injected TIIP (AS-TIIP) by western blotting. The protein expression of TRPV1 did not change in pH 7.0-treated TIIP and AS- TIIP rat groups compared to 7.0-treated sham groups (n = 4 in each group, 3 days after surgery and pH 4.0 injection). Each band was normalized against the corresponding β-actin band used as loading control. (B) We detected PKC dependent pTRPV1 using western blotting in the hind paw skin of sham + pH 7.0 saline, sham + pH 4.0 saline, TIIP + pH 7.0 saline and AS-TIIP (n = 4 in each group, 3 days after surgery and pH 4.0 saline injections). The expression of pTRPV levels significantly increased in AS-TIIP rats (**p < 0.01 vs sham + pH 7.0 saline) but not in pH 4.0 treated sham and pH 7.0-treated TIIP rats. The pTRPV1 level was normalized against corresponding total TRPV1. Data are presented here as a percentage change (%) compared to the sham control.

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