Skip to main content
Figure 4 | Molecular Pain

Figure 4

From: Acid evoked thermal hyperalgesia involves peripheral P2Y1 receptor mediated TRPV1 phosphorylation in a rodent model of thrombus induced ischemic pain

Figure 4

The effect of acid in thermal hyperalgesia (TH) and TRPV1 expression in Resiniferatoxin (RTX) treated rats. (A) Immunofluorescent images of rat DRG neurons. Thin sections (10 μm) of rat DRGs (L4-L5) were stained with antibodies against TRPV1 in the vehicle and RTX treated groups. The TRPV1-immunostaining in DRG neurons were almost completely abolished in RTX treated rats. Images are shown at 200× magnification. Scale bars represent 100 μm. (B) Short-term effects of acid induced TH in RTX treated TIIP rats at day 3. We evaluated the development of TH in RTX treated sham + pH 4.0 and acidic saline (pH 4.0) injected TIIP (AS-TIIP) rats (n = 4 in each group) at 30 min, 1, 2, 4, 8 and 12 hours after saline injection. RTX treated rats did not show TH following TIIP surgery and pH 4.0 injection. (C) TRPV1 receptor expression in hind paw skin lysates was quantitatively evaluated in RTX treated groups including sham + pH 7.0 saline, sham + pH 4.0 saline, TIIP + pH 7.0 saline and AS-TIIP by western blotting. The protein expression of TRPV1 did not change in any of the RTX treated groups compared to 7.0-treated sham groups (n = 6 in each group, 3 days after surgery and pH 4.0 injection). Each band was normalized against the corresponding β-actin band used as loading control. (D) pTRPV1 expression in hind paw skin lysates was quantitatively evaluated in RTX treated group including sham + pH 7.0 saline, sham + pH 4.0 saline, TIIP + pH 7.0 saline and AS-TIIP by western blotting. The protein expression of pTRPV1 did not change in any of the RTX treated groups compared to 7.0-treated sham groups (n = 6 in each group, 3 days after surgery and pH 4.0 injection). The pTRPV1 level was normalized against corresponding total TRPV1. Data are presented here as a percentage change (%) compared to the sham control.

Back to article page