Table 2 Unique sequences of miRNA isoforms (isomiRs) and other sncRNAs (with RPM ≥100) that underwent significant (qFDR < 0.05) differential up- or down-regulation following SNL nerve injury in HA vs. LA rats
Small RNA type and sequence | Mean RPM | Mean RPM | SNL-reg | diff-reg | |||
|---|---|---|---|---|---|---|---|
(canonical miRNA or RNA type) | (sham pools) | (SNL pools) | subtraction method (SNL/sham fold-change) | Â | |||
| Â | HA | LA | HA | LA | HA | LA | HA-LA |
unique-sequence miRNA isoform (isomiR), all pools, and those added by HA_SNL1 pool exclusion * | |||||||
UAUAGUACUGUGAUAACUGACU (rno-miR-101a-3p) | 11.7 | 9.3 | 9.9 | 11.3 | −0.167 (−1.182) | 0.194 (1.215) | −0.361 (0.397) |
CAGUGCAAUGUUAAAAGGGC (rno-miR-130a-3p) * | 38.0 | 41.9 | 48.2 | 45.0 | 0.237 (1.268) | 0.071 (1.074) | 0.166 (0.194) |
UCCCUGAGACCCUAACUUG (rno-miR-125b-5p) * | 47.8 | 30.4 | 35.5 | 33.1 | −0.295 (−1.346) | 0.085 (1.089) | −0.380 (0.435) |
UGGACGGUGUGAGGCC (sha-miR- 5105) * | 11.0 | 15.6 | 13.5 | 11.9 | 0.204 (1.227) | −0.269 (−1.311) | 0.473 (0.537) |
unique-sequence sncRNAs with RPM ≥100, after HA_SNL1 pool exclusion | |||||||
AGCCGCCUGGAUACCGCAGCUAGGAAUAA (rRNA) | 1843.3 | 616.5 | 548.0 | 666.0 | −1.846 (−3.363) | 0.078 (1.080) | −1.924 (2.443) |
GCAUUAGUGGUUCAGUGG (tRNA) | 621.1 | 719.9 | 771.1 | 713.5 | 0.215 (1.242) | −0.009 (−1.009) | 0.224 (0.251) |
UCAUUGGUGGUUCAGUGG (tRNA) | 88.6 | 111.5 | 114.1 | 103.4 | 0.252 (1.288) | −0.075 (−1.078) | 0.327 (0.366) |