Figure 1

Chemerin attenuates capsaicin potentiation of mEPSC frequency in lamina I NK1R+ neurons. A and D. Representative mEPSC traces recorded before (top) and during (bottom) capsaicin (1 μ M) application in the absence (A) and presence (D) of chemerin (100 ng/ml). B and E. Example cumulative probability plots demonstrate the significant leftward shift in mEPSC inter-event intervals that results from capsaicin applied alone (B) or in the presence of chemerin (E) (both P < 0.00001, Kolmogorov-Smirnov 2-sample test). C and F. Application of capsaicin significantly increases mEPSC frequency when applied alone (C, P = 0.002, paired t-test, n = 12) and during chemerin application (F, P = 0.008, paired t-test, n = 10). G. Capsaicin significantly increases mEPSC frequency in the presence and absence of chemerin (P = 0.0001, 2-way repeated measures ANOVA), however this increase is significantly attenuated by chemerin (P = 0.031, Bonferroni post-tests). All data presented as mean ± SEM, grey points and lines in C and F indicate trajectories for individual neurons. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.