Figure 3From: The chemerin receptor 23 agonist, chemerin, attenuates monosynaptic C-fibre input to lamina I neurokinin 1 receptor expressing rat spinal cord neurons in inflammatory pain Primary afferent fibre characterisation in compound action potential recordings. A. Representative compound action potential trace recorded from an isolated dorsal root, evoked by 500 μ A stimulation, illustrating the fast (A β), medium (A δ) and slow (C) conducting components (average of 10 traces shown). B. Threshold stimulus intensity of the A β-, A δ- and C-fibre components, which differed significantly with fibre type (P < 0.0001, 1-way ANOVA, n = 9). C. Stimulus response relationship for the C-fibre component. D. 1 Hz stimulation of an isolated dorsal root resulted in a prolongation of latency in the C-, but not A δ-fibre component. Broken lines denote the negative peak of the A δ-/C-fibre response during the first and last stimuli. E. Quantification of the latency prolongation demonstrated that the C-fibre, but not A δ-fibre, component exhibited a significant frequency dependent latency prolongation (A δ vs. C and 1 Hz vs. 2 Hz, P < 0.0001, 2-way ANOVA). All data presented as mean ± SEM. Dotted lines in B. indicate the dorsal root stimulation intensities (A β, 20 μ A; A δ, 100 μ A; C, 500 μ A) used in the subsequent patch-clamp electrophysiology studies. ∗∗∗∗ P < 0.0001.Back to article page