Figure 7

Chemerin attenuates C-fibre input to a subset of lamina I NK1R+ neurons in CFA inflammation. A. Chemerin has no effect on the peak amplitude of monosynaptic C-fibre eEPSCs in the population of neurons classified as ‘non-responders’ (P = 0.802, 2-way repeated measures ANOVA, representative traces shown in B). In this subgroup of non-responders, chemerin did not alter the distribution of actual C-fibre eEPSC peak amplitudes (P = 0.983, paired t-test, C). D. In the subgroup of chemerin responders, chemerin significantly reduces the C-fibre eEPSC amplitude (P = 0.001, 2-way repeated measures ANOVA, representative traces shown in E). Furthermore, in neurons classified as chemerin responders, chemerin resulted in a significant leftward shift in the distribution of actual C-fibre eEPSC peak amplitudes (P = 0.028, paired t-test, F). Example traces show an average of 3 sweeps, baseline and chemerin traces recorded at points ‘a.’ and ‘b.’ respectively, denoted by arrows on relevant graphs. Vehicle n = 7, chemerin responders n = 7, chemerin non-responders n = 9. Data displayed as mean ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.