Spinal nerve injury increases the expression of Iba-1 and GFAP: Blockade by minocycline or fluorocitrate. Effect of the repeated intrathecal administration of minocycline (Mino, panel A and B) or fluorocitrate (Fluo, panel C and D) in spinal nerve injury-induced increased expression of Iba-1 and GFAP in the ipsilateral (IL) and contralateral (CL) dorsal spinal cord. Minocycline (panel E) and fluorocitrate (panel F) also reduced tactile allodynia in spinal nerve ligated rats. Data were normalized against β-actin and are expressed as the mean ± S.E.M. of 3 independent rats. * Significantly (p < 0.05) different from the sham (S) or naïve (N) groups and # significantly (p < 0.05) different from the spinal nerve ligated (SNL) group, as determined by one-way analysis of variance followed by the Student-Newman-Keuls test.