nAChR subunit | Fold change microrarray | Fold change RT-PCR |
---|
 | TG | TG | DRG |
---|
α2 | 1.06 | ND | −1.38* |
α3 | 4.84 | 13.35* | 3.98* |
α4 | 0.67, 1.02 | NC |
α5 | 0.9 | NC |
α6 | 1.38 | NC |
α7 | 1.05 | NC |
α9 | 1.06 | NC |
β2 | 0.92, 0.96, 1.01 | NC |
β3 | 3.13, 3.2, 2.06 | 4.08* | NC |
β4 | 1.56 | 7.68* | 2.82* |
- Microarray data comparing ART-OE (n = 3) and WT (n = 3) mice was validated using SYBR green qRT-PCR assay. Multiple values in array data indicate number of times gene was represented on array. RT-PCR assays analyzed RNA isolated from 3–6 mice. NC = not changed, ND = not done. Asterisk indicates p <0.05.