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Table 1 Expression of nAChR subunits in ART-OE trigeminal (TG) and lumbar DRG

From: Artemin growth factor increases nicotinic cholinergic receptor subunit expression and activity in nociceptive sensory neurons

nAChR subunit

Fold change microrarray

Fold change RT-PCR

 

TG

TG

DRG

α2

1.06

ND

−1.38*

α3

4.84

13.35*

3.98*

α4

0.67, 1.02

NC

α5

0.9

NC

α6

1.38

NC

α7

1.05

NC

α9

1.06

NC

β2

0.92, 0.96, 1.01

NC

β3

3.13, 3.2, 2.06

4.08*

NC

β4

1.56

7.68*

2.82*

  1. Microarray data comparing ART-OE (n = 3) and WT (n = 3) mice was validated using SYBR green qRT-PCR assay. Multiple values in array data indicate number of times gene was represented on array. RT-PCR assays analyzed RNA isolated from 3–6 mice. NC = not changed, ND = not done. Asterisk indicates p <0.05.