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Figure 1 | Molecular Pain

Figure 1

From: Postsynaptic potentiation of corticospinal projecting neurons in the anterior cingulate cortex after nerve injury

Figure 1

Fos/FG double labeling after Fluoro-Gold injection into the spinal cord. A, Schematic figures and digitized photomicrograph showing Fluoro-Gold (FG) injection site in the spinal cord and retrograde transportation of FG label neurons in the ACC. B, Distribution of FG labeled neurons in both sides of ACC after FG injection into the spinal cord. C, D, Augmented figures showing FG (green) and Fos (red) double-labeling results in rectangle area 1 (C) and 2 (D) in B. Arrowheads on the merged figures indicate FG/Fos double-labeled neurons. E, After FG injection into one side of the dorsal part of spinal cord, the FG retrogradely labeled cells in the ACC was immunostained with anti-FG antibody and shown with ABC method. With Nissl counterstaining, most of the FG labeled cells are shown to be located within the layer V of the ACC. F, Augmented figures from upper (left) and lower (right) rectangled areas in E showed FG labeled neurons located in the layer V of the ACC. Bars equal to 200 μm in A, B and E and 20 μm in C, D and F.

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