Figure 6

GluA1/3 mediated the potentiated input–output responses in ACC-spinal cord projecting neurons. A, Digitized photomicrograph showing one DiI retrogradely labeled neuron after injection into the spinal cord (SC) was whole-cell patched and dual labeled by intracellular injection of Alex-488 (1), and one Alex-488 Dextran retrogradely labeled neuron after injection into the ventral striatum (VS) was patched and dual labeled with Alex-594(2). B, Samples and summarized results showing the I-O curve in ACC-SC projecting neurons in mice with nerve injury has steeper slope, as compared with ACC-SC projecting neurons in mice with sham surgery. Meanwhile, the I-O curve in ACC-VS projecting neurons was not different in mice with or without nerve injury. C, Bath application of NASPM only inhibited the I-O responses of neurons from nerve injury but not sham surgery group. D, Bath application of NASPM only inhibited the I-O responses of ACC-SC projecting neurons from nerve injury but not sham surgery group. E, Bath application of NASPM inhibited the I-O responses of ACC-VS projecting neurons from neither nerve injury nor sham surgery group. F. Nerve injury enhanced the I-O responses of ACC-SC projecting neurons in s831A mice but not in s845A mice. Bar equals to 20 μM in A. *,p < 0.05; ***,p < 0.001.