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Figure 4 | Molecular Pain

Figure 4

From: Spinal 5-HT3 receptors mediate descending facilitation and contribute to behavioral hypersensitivity via a reciprocal neuron-glial signaling cascade

Figure 4

Attenuation of SR57227-induced upregulation of fractalkine receptor CX3CR1 in spinal microglia and behavioral hypersensitivity to a neutralizing antibody against CX3CR1. A. CX3CR1 immunoreactivity exists in all dorsal horn microglial cells (arrows) expressing CD11b at 2 h after intrathecal SR57227 (10 pmol). Scale bar = 25 μm. B. Western blot analysis showing a significant upregulation in the levels of CX3CR1 in the spinal dorsal horn tissue in rats at 1 and 2 h after intrathecal injection of SR57227 (10 pmol) but not vehicle saline (*, p < 0.05, vs. saline, n = 3 per group). C. SR57227-induced increases in the protein levels of Iba1 in the spinal dorsal horn tissue were significantly blocked by a neutralizing antibody against CX3CR1 (CX3CR1 Ab, 20 μg) at 2 h after co-administration (*, p < 0.05, vs. saline + saline; #, p < 0.05, CX3CR1 Ab + SR57227 vs. saline + SR57227; n = 3 per group). D. Thermal (left) and mechanical (right) hypersensitivity induced by SR57227 (10 pmol, i.t.) was partially eliminated by pretreatment with a CX3CR1 neutralizing antibody (Ab) (20 μg, i.t.) 1 d before and concurrently with SR57227 at 2 h after injection of SR 57227. *, p < 0.05, vs. saline + saline; #, p < 0.05, CX3CR1 Ab + SR57227 vs. saline + SR57227, n = 5 per group).

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Molecular Pain

ISSN: 1744-8069

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