| Yo-Pro-1 uptake | Ca 2+flux | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
WT | 155Tyr | 348Thr | 496Ala | 496Ala 155Tyr | WT | 155Tyr | 348Thr | 496Ala | 496Ala 155Tyr | |
EC50 (uM) | 46.64 ± 5.32 | 56.65 ± 2.05 | 45.24 ± 0.54 | 34.56 ± 5.12 | 53.18 ± 5.28 | 67.48 ± 2.1 | 71.04 ± 4.6 | 58.22 ± 1.54 | 96.66 ± 5.88 (*) | 89.23 ± 3.45 (**) |
Hill slope | 1.27 ± 0.10 | 1.72 ± 0.04 | 1.77 ± 0.24 | 1.2 ± 0.51 | 2.01 ± 0.47 | 2.77 ± 0.25 | 2.52 ± 0.17 | 2.67 ± 0.42 | 2.67 ± 0.28 | 2.73 ± 0.34 |
Efficacy (%) | 100 | 177.68 ± 6.18 (*) | 218.06 ± 19.49 (*) | 23.56 ± 2.5 (*) | 33.46 ± 5.78 (*) | 100 | 125.83 ± 11.74 (*) | 137.23 ± 16.06 (*) | 46.2 ± 6.87 (*) | 54.14 ± 3.6 (*) |
n | 13 | 3 | 4 | 3 | 3 | 15 | 3 | 3 | 6 | 3 |