Attenuation of mechanical hypersensitivity by 5,7-DHT treatment. A). Attenuation of mechanical hypersensitivity in 5,7-dihydroxytryptamine treated rats following ankle joint inflammation. Ipsilateral paw withdrawal thresholds were significantly higher in 5,7-DHT treated animals compared with saline controls, at 1d and 2d following CFA injection. Pre-IT indicates the baseline prior to intrathecal injection, post-IT indicates baseline after IT injection. B). Paw withdrawal thresholds of the contralateral hindpaw were not altered by CFA injection or by 5,7-DHT pre-treatment. Data is presented as log2 (paw withdrawal threshold in g) and mean ± SEM. Line indicates significant 5,7-DHT x CFA interaction in ANOVA, * indicates p < 0.05, LSD post hoc tests. n = 6–7 per group.