Figure 2

Expression of Grp mRNA in DRGs. A) Grp mRNA was detected in WT and BRAF^Nav1.8 DRGs using ISH. Scale bars: 100 μm. B) Real-time RT-PCR showed that Grp mRNA level was dramatically enhanced in BRAF^Nav1.8 DRG. n = 4. *p < 0.0001 versus WT, unpaired t test. C) Validation of Grp primers using real-time RT-PCR and a series of amounts of DRG cDNA (0.01, 0.1 and 1 μl). The reactions were specific because there were no detectable signals when DRG cDNA was substituted with H₂O (blue) or ∆RT control (red) in which reverse transcriptase was omitted. D) Melt curve to show that Grp PCR product has a single narrow peak at 83°C.