Figure 7
Protein kinase Cϵ (PKCϵ) does not contribute to nociceptor priming in acid-induced muscle pain model. (A-D) Effect of PKCϵ inhibitor peptide (V1–2, EAVSLKPT) on acid-induced hyperalgesia. Intramuscular injection of neutral saline (A, C) or 1 nmole PKCϵ inhibitor peptide (B, D) 5 hr after the first acid injection (A, B) or 3 min before the second acid injection (C, D) did not affect the development of hyperalgesia. (E-H) Effect of a general PKC inhibitor (BIM) on acid-induced hyperalgesia. Intramuscular injection of neutral saline (E, G) or 9.7 nmole BIM (F, H) 5 hr after the first acid injection (E, F) or 3 min before the second acid injection (G, H) did not affect the development of hyperalgesia. Black arrows indicate when mice received the intramuscular acid injection. B, baseline on day 0; D, day. *P < 0.05 compared with the response before the second acid injection.