TRPC family gene expression in sensory ganglia. (A) TRPC mRNA detection using a RT-PCR screen against TRPC1-7 in adult rat DRG. TRPC1 and TRPC3 are the major subunits expressed in DRG, along with low levels of TRPC6 and little or no signal for TRPC 2, 4, 5 and 7. (B) In situ expression profile of all TRPC family members at the whole DRG and spinal cord level. TRPC1 and TRPC3 are expressed at high levels in DRGs, with minimal detection in the spinal cord. Low but significant TRPC6 expression is also observed at the DRG level. (C) Emulsion staining with cellular resolution clearly shows that TRPC3 mRNA is localized in a subpopulation of small and medium diameter neurons (arrows) in DRG and trigeminal ganglia (TGG). Representative negative small-diameter and large-diameter neurons are also indicated (stars). Scale bar = 50 μm.