Figure 3

Disruption of CREB action in the DRG attenuates IL-6-induced nociceptive plasticity. A) Intrathecal injection of cAMP response element (CRE) consensus sequence DNA oligonucleotides at the same time as intraplantar IL-6, prevented the development of IL-6-induced mechanical hypersensitivity and hyperalgesic priming. Mutant CRE oligos were not effective. N = 6 mice per condition. B) Intrathecal injection of CRE consensus sequence DNA oligonucleotide (CRE con) at the same time as intraplantar IL-6, prevented enhancement of BDNF expression in L4-L6 DRGs 3 hours following injections. N = 6 mice per condition. C) Intrathecal injection of BDNF (0.1 ng) mechanical hypersensitivity and hyperalgesic priming. Co-injection of CRE consensus sequence DNA oligonucleotides with BDNF did not affect mechanical hypersensitivity and hyperalgesic priming. N = 6 mice per condition. * = p < 0.05, ** = p < 0.01 and *** = p < 0.001.