Figure 2

Profiling the gene expression alterations following minocycline i.p. administration to CCI-exposed rats. Gene expression changes 7 days after nerve injury and administration of minocycline (30 mg/kg; i.p.; 16 h and 1 h before CCI and then for 7 days twice daily). The statistical analysis was performed at an ANOVA threshold value of p < 0.001 (N vs. V-CCI vs. MC-CCI). The results of the cDNA microarray analysis are presented in the form of a heat map. The individual columns represent the respective microarrays, while the individual rows represent the regulated genes. The intensity of the color reflects the relative abundance of the transcript and is proportional to the standard deviation from the in-row average, as indicated in the legend below the heat map. (A) The gene pattern represents changes in the abundance levels of 54 mRNA transcripts induced in the lumbar spinal segment on day 7 of neuropathic pain; these abundance levels of these genes were not affected by the addition of minocycline (30 mg/kg; 16 h and 1 h before sciatic nerve damage and then twice daily for 7 days). (B) Gene pattern B represents changes in the abundance levels of 39 mRNA transcripts induced in the lumbar spinal segment on day 7 of neuropathic pain; the abundance levels of these genes were modulated by repeated intraperitoneal administration of minocycline (30 mg/kg; 16 h and 1 h before sciatic nerve damage and then twice daily for 7 days). (C) The percentage analysis of the alterations in the CCI-induced gene expression profile in the spinal cord.