Figure 1
From: Anoctamin 1 contributes to inflammatory and nerve-injury induced hypersensitivity
Tissue specific ablation of Ano1 in DRG neurons reduces inflammatory thermal and mechanical hyperalgesia. (A) Detection of ANO1, Nav1.8 and TRPV1 mRNAs in mouse DRG neurons of Ano1fl/fl (control; CTL) and Adv/Ano1fl/fl (conditional knockout; CKO) mice using reverse transcription-PCR. M, DNA molecular weight markers. (B) Time courses of the withdrawal latency to thermal stimuli were examined in Adv/Ano1fl/fl and its control mice 30, 60, 90 120, 150 min, 24 hr after carrageenan injection (2%, 50 μl). Radiant heat stimulus (IR 50) was applied to left paws of Adv/Ano1fl/fl and its control mice (n = 7–8, *p < 0.05, **p < 0.01, ***p < 0.001). Basal withdrawal latency was measured before the injection of carrageenan. (C) Time courses of withdrawal thresholds of Adv/Ano1fl/fl and its control mice to Von Frey hair stimuli 30, 60, 90 120, 150 min, 24 hr after carrageenan injection. Mechanical stimuli were applied to left hind paws of Adv/Ano1fl/fl and its control mice with Von Frey hairs (n = 6, **p < 0.01, ***p < 0.001). Basal withdrawal threshold was measured before the injection of carrageenan.