Figure 3

Ablation of ANO1 in DRG neurons reduces spinal nerve injury-induced thermal and mechanical hyperalgesia. (A) Neuropathic pain was induced on a hind paw after dissecting two out of three branches in the sciatic nerve. Paw withdrawal latencies of ipsilateral (Ipsi) and contralateral (contra) hind paws of Adv/Ano1^fl/fl and control mice were measured in response to radiant heat after spared nerve injury (*p < 0.05, ***p < 0.001 compared to the withdrawal latency of ipsilateral hind paw of control mice, n = 5–6). Basal paw withdrawal latency was measured before the surgery. (B) Withdrawal thresholds of ipsilateral and contralateral hind paws of Adv/Ano1^fl/fl and control mice were measured with Von Frey hairs after spared nerve injury (*p < 0.05, **p < 0.01 compared to the withdrawal threshold of ipsilateral hind paw of control mice, n = 5–6).